MVIIA (1386) Protein Card

General Information

Name	MVIIA
Alternative name(s)	M7a,SNX-111,Ziconotide
Organism	Conus magus (Magus cone)
Organism Region	Indo-Pacific
Organism Diet	piscivorous
Protein Type	Wild type
Protein precursor	MVIIA precursor (1564)

Classification

Class	conotoxin
Gene superfamily	O1 superfamily
Cysteine framework	VI/VII [connectivity I-IV, II-V, III-VI]
Pharmacological family	omega conotoxin

Sequence

CKGKGAKCSRLMYDCCTGSCRSGKC(nh2)
Modified residues	position symbol name 26 nh2 C-term amidation
Sequence evidence	protein level
Average Mass	2639.12
Monoisotopic Mass	2637.10
Isoelectric Point	11.55
Extinction Coefficient [280nm]	1490.00

References

Olivera,B.M., Gray,W.R., Zeikus,R., McIntosh,J.M., Varga,J., Rivier,J., de Santos,V. and Cruz,L.J. (1985) Peptide neurotoxins from fish-hunting cone snails Science 230:1338-1343

Olivera,B.M., Cruz,L.J., de Santos,V., Lecheminant,G.W., Griffin,D., Zeikus,R.D., McIntosh,J.M., Galyean,R., Varga,J., Gray,W.R. and Rivier,J.E. (1987) Neuronal calcium channel antagonists. Discrimination between calcium channel subtypes using omega-conotoxin from Conus magus venom Biochemistry 26:2086-2090

Kohno,T., Kim,J.I., Kobayashi,K., Kodera,Y., Maeda,T. and Sato,K. (1995) Three-dimensional structure in solution of the calcium channel blocker omega-conotoxin MVIIA Biochemistry 34:10256-10265

Nielsen,K.J., Thomas,L., Lewis,R.J., Alewood,P.F. and Craik,D.J. (1996) A consensus structure for omega-conotoxins with different selectivities for voltage-sensitive calcium channel subtypes: comparison of MVIIA, SVIB and SNX-202 J. Mol. Biol. 263:297-310

Atkinson,R.A., Kieffer,B., Dejaegere,A., Sirockin,F. and Lefevre,J.F. (2000) Structural and dynamic characterization of omega-conotoxin MVIIA: the binding loop exhibits slow conformational exchange Biochemistry 39:3908-3919

Adams,D.J., Smith,A.B., Schroeder,C.I., Yasuda,T. and Lewis,R.J. (2003) Omega-conotoxin CVID inhibits a pharmacologically distinct voltage-sensitive calcium channel associated with transmitter release from preganglionic nerve terminals J. Biol. Chem. 278:4057-4062

Kim,J.I., Takahashi,M., Martin-Moutot,N., Seagar,M.J., Ohtake,A. and Sato,K. (1995) Tyr13 is essential for the binding of omega-conotoxin MVIIC to the P/Q-type calcium channel Biochem. Biophys. Res. Commun. 214:305-309

Mould,J., Yasuda,T., Schroeder,C.I., Beedle,A.M., Doering,C.J., Zamponi,G.W., Adams,D.J. and Lewis,R.J. (2004) The alpha2delta auxiliary subunit reduces affinity of omega-conotoxins for recombinant N-type (Cav2.2) calcium channels J. Biol. Chem. 279:34705-34714

Internal links

Protein Precursor	MVIIA precursor (1564)
Nucleic acids
Structure	NMR STRUCTURE OF OMEGA-CONOTOXIN MVIIA: CONSTRAINTS ON DISULPHIDE BRIDGES NMR STRUCTURE OF OMEGA-CONOTOXIN MVIIA: NO CONSTRAINTS ON DISULPHIDE BRIDGES N-TYPE CALCIUM CHANNEL BLOCKER, OMEGA-CONOTOXIN MVIIA, NMR, 15 STRUCTURES NMR STUDY OF OMEGA-CONOTOXIN MVIIA 1OMG 3 NMR SOLUTION STRUCTURE OF OMEGA-CONOTOXIN MVIIA, A N-TYPE CALCIUM CHANNEL BLOCKER

External links

UniProtKB/Swiss-Prot	P05484
Ncbi	159162794 159162717 159162716 50513808 15988525 8569683 2392461 1421016 253723035 253723010

Tools

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Please cite: Kaas Q, Westermann JC, Halai R, Wang CK and Craik DJ. ConoServer, a database for conopeptide sequences and structures. Bioinformatics (2008) 24(3):445-6

ConoServer is managed at the Institute of Molecular Bioscience IMB, Brisbane, Australia.

Data and tools found on this website may be used for academic research only, provided that it is referred to ConoServer, the database of conotoxins (http://www.conoserver.org). For any other use please contact David Craik (d.craik@imb.uq.edu.au).

Contacts:
David Craik
Quentin Kaas

Last updated: Tuesday 24 November 2009