De Novo Sequencing
Genome sequencing technology continues to advance with ever increasing throughput and reducing costs. A major leap in this technology has recently occurred with the development of massively parallel sequencing methods commercialised by Roche, Solexa, and Applied Biosystems. These methods differ from traditional Sanger sequencing technology in that they produce very large numbers of relatively short sequence reads. The short read sequencing approach has been applied successfully for the rapid and cost effective sequencing of genomes where sequence assembly has been facilitated by comparison with closely related available genome sequences (re-sequencing). The complexity and size of the majority of eukaryotic genomes, has so far limited de novo genome sequencing using these new methods. We aim develop and validate an approach for the de novo sequencing of complex eukaryotic genomes using short read sequencing by applying novel bioinformatics tools.